Geometric modeling and processing
Ambito disciplinare
-
A CAD/CAE integration framework for analyzing and designing spatial compliant mechanisms via pseudo-rigid-body methods
Robotics and Computer-Integrated Manufacturing, 56, pp. 287-302
Abstract
Compliant Mechanisms (CMs) are currently employed in several engineering applications requiring high precision and reduced number of parts. For a given mechanism topology, CM analysis and synthesis may be developed resorting to the Pseudo–Rigid Body (PRB) method, in which the behavior of flexible members is approximated via a series of rigid links connected by spring-loaded kinematic pairs. From a CM analysis standpoint, the applicability of a generic PRB model requires the determination of the kinematic pairs' location and the stiffness of a set of generalized springs. In parallel, from a design standpoint, a PRB model representing the kinetostatic behavior of a flexible system should allow to compute the flexures' characteristics providing the desired compliance. In light of these considerations, this paper describes a Computer-Aided Design/Engineering (CAD/CAE) framework for the automatic derivation of accurate PRB model parameters, on one hand, and for the shape optimization of complex-shape flexures comprising out-of-plane displacements and distributed compliance. The method leverages on the modelling and simulation capabilities of a parametric CAD (i.e. PTC Creo) seamlessly connected to a CAE tool (i.e. RecurDyn), which provides built-in functions for modelling the motion of flexible members. The method is initially validated on an elementary case study taken from the literature. Then, an industrial case study, which consists of a spatial crank mechanism connected to a fully-compliant four-bar linkage is discussed. At first, an initial sub-optimal design is considered and its PRB representation is automatically determined. Secondly, on the basis of the PRB model, several improved design alternatives are simulated. Finally, the most promising design solution is selected and the dimensions of a flexure with non-trivial shape (i.e. hybrid flexure) is computed. This technique, which combines reliable numerical results to the visual insight of CAD/CAE tools, may be particularly useful for analyzing/designing spatial CMs composed of complex flexure topologies.
Keywords: CAD/CAE integration , Distributed compliance , Pseudo rigid body models , Shape optimization , Spatial compliant mechanisms
-
Re-design of a packaging machine employing linear servomotors: A description of modelling methods and engineering tools
Procedia Manufacturing, 38, pp. 784-791
Abstract
Position-controlled servo-systems mostly make use of electric rotary motors and gearboxes and, if necessary, a transmission mechanism to convert rotary into linear motion. Even so, especially in the field of automatic machines for packaging, it should be highlighted that most of the required movements are usually linear, so that Linear Electric Motors (LEM) should somehow represent a more convenient solution for designers. LEM can directly generate the required trajectory avoiding any intermediate mechanism, thus potentially minimizing the number of linkages/mechanical parts and, therefore, the undesired backlash and compliance that come along. On the other hand, particularly within small-medium enterprises, LEM may be rarely employed despite obvious advantages, mostly due to their high-cost as compared to rotary actuators and the lack of knowledge of the achievable performance. In light of these considerations, the present paper reports an industrial case study where an automatic machine for packaging, comprising distributed actuation and several tasks requiring a linear motion, has been completely redesigned employing different kind of LEM (i.e. iron-core and iron-less). Such machine architecture is compared to a “traditional†design where brushless gearmotors are coupled to linkage systems. The paper mainly focuses on the selection criteria for the LEM system and on the engineering tools employed during the different design stages. Qualitative and quantitative conclusions are finally drawn, which may provide useful hints for designers that are willing to actually employ LEM-based solutions in an industrial scenario.
Keywords: CAD/CAE , Engineering Design Methods and Tools for Industry 4.0 , High Precision Manufacturing , Modelling , Simulation
-
Customized Cutting Template to Assist Sternotomy in Pectus Arcuatum
Annals of Thoracic Surgery, 107(4), pp. 1253-1258
3D scanningMethods for medical applications
Abstract
Purpose: Pectus arcuatum is an anterior chest wall deformity that requires transverse wedge sternotomy. Determining and delivering the correct cutting angle are crucial for successful correction. This report describes the early clinical experience with a novel cutting template technology able to deliver the optimal cutting angle. Description: From patients' computed tomographic scans, the optimal cutting angle is obtained using computer-aided design. A template comprising slots tilted at the right cutting angle and a safety block to avoid damaging the posterior periosteum is printed through additive manufacturing. Evaluation: The template allows surgeons to perform a precise wedge sternotomy, safely sparing the posterior periosteum in all patients, without complications. Postoperative chest roentgenograms and clinical photographs demonstrate optimal sternal realignment and cosmetic outcome. In this report, the mean operative time was 110 minutes. All patients were successfully discharged, with a mean length of stay of 4 days. Conclusions: Transverse wedge sternotomy aided by a computer-aided design–devised cutting template may reduce the technical challenge of this procedure, thereby increasing its safety and reducing operative times and hospital stay. Further research on long-term patient outcomes is necessary.
-
Metrological and critical characterization of the intel D415 stereo depth camera
Sensors (Switzerland), 19(3)
Abstract
Low-cost RGB-D cameras are increasingly being used in several research fields, including human–machine interaction, safety, robotics, biomedical engineering and even reverse engineering applications. Among the plethora of commercial devices, the Intel RealSense cameras have proven to be among the most suitable devices, providing a good compromise between cost, ease of use, compactness and precision. Released on the market in January 2018, the new Intel model RealSense D415 has a wide acquisition range (i.e., ~160–10,000 mm) and a narrow field of view to capture objects in rapid motion. Given the unexplored potential of this new device, especially when used as a 3D scanner, the present work aims to characterize and to provide metrological considerations for the RealSense D415. In particular, tests are carried out to assess the device performance in the near range (i.e., 100–1000 mm). Characterization is performed by integrating the guidelines of the existing standard (i.e., the German VDI/VDE 2634 Part 2) with a number of literature-based strategies. Performance analysis is finally compared against the latest close-range sensors, thus providing a useful guidance for researchers and practitioners aiming to use RGB-D cameras in reverse engineering applications.
Keywords: Active stereo , Depth camera , Device characterization , Performance comparison , RealSense D415 , Reverse engineering , VDI/VDE standard
-
Towards a CAD-based automatic procedure for patient specific cutting guides to assist sternal osteotomies in pectus arcuatum surgical correction
Journal of Computational Design and Engineering, 6(1), pp. 118-127
Additive manufacturingMethods for medical applicationsProduct design and engineering
Abstract
Pectus Arcuatum, a rare congenital chest wall deformity, is characterized by the protrusion and early ossification of sternal angle thus configuring as a mixed form of excavatum and carinatum features. Surgical correction of pectus arcuatum always includes one or more horizontal sternal osteotomies, consisting in performing a V-shaped horizontal cutting of the sternum (resection prism) by means of an oscillating power saw. The angle between the saw and the sternal body in the V-shaped cut is determined according to the peculiarity of the specific sternal arch. The choice of the right angle, decided by the surgeon on the basis of her/his experience, is crucial for a successful intervention. The availability of a patient-specific surgical guide conveying the correct cutting angles can considerably improve the chances of success and, at the same time, reduce the intervention time. The present paper aims to propose a new CAD-based approach to design and produce custom-made surgical guides, manufactured by using additive manufacturing techniques, to assist the sternal osteotomy. Starting from CT images, the procedure allows to determine correct resection prism and to shape the surgical guide accordingly taking into account additive manufacturing capabilities. Virtually tested against three case studies the procedure demonstrated its effectiveness.
Keywords: Biomedical devices , CAD , Design for additive manufacturing , Medical imaging
-
Modeling, assessment, and design of porous cells based on schwartz primitive surface for bone scaffolds
Scientific World Journal, 2019
Methods for medical applications
Abstract
Te design of bone scafolds for tissue regeneration is a topic of great interest, which involves diferent issues related to geometry of architectures, mechanical behavior, and biological requirements, whose optimal combination determines the success of an implant. Additive manufacturing (AM) has widened the capability to produce structures with complex geometries, which should potentially satisfy the diferent requirements. These architectures can be obtained by means of refned methods and have to be assessed in terms of geometrical and mechanical properties. In this paper a triply periodic minimal surface (TPMS), the Schwarz's Primitive surface (P-surface), has been considered as scafold unit cell and conveniently parameterized in order to investigate the efect of modulation of analytical parameters on the P-cell geometry and on its properties. Several are the cell properties, which can afect the scafold performance. Due to the important biofunctional role that the surface curvature plays in mechanisms of cellular proliferation and diferentiation, in this paper, in addition to properties considering the cell geometry in its whole (such as volume fraction or pore size), new properties were proposed. Tese properties involve, particularly, the evaluation of local geometrical-diferential properties of the P-surface. Te results of this P-cell comprehensive characterization are very useful for the design of customized bone scafolds able to satisfy both biological and mechanical requirements. A numerical structural evaluation, by means of fnite element method (FEM), was performed in order to assess the stifness of solid P-cells as a function of the changes of the analytical parameters of outer surface and the thickness of cell. Finally, the relationship between stifness and porosity has been analyzed, given the relevance that this property has for bone scafolds design.
-
Performance evaluation of underwater image pre-processing algorithms for the improvement of multi-view 3D reconstruction
Acta IMEKO, 8(3), pp. 69-77
3D scanning3D technologies for cultural heritages
Abstract
3D models of submerged structures and underwater archaeological finds are widely used in various and different applications, such as monitoring, analysis, dissemination, and inspection. Underwater environments are characterised by poor visibility conditions and the presence of marine flora and fauna. Consequently, the adoption of passive optical techniques for the 3D reconstruction of underwater scenarios is a highly challenging task. This article presents a performance analysis conducted on a multi-view technique that is commonly used in air in order to highlight its limits in the underwater environment and then provide guidelines for the accurate modelling of a submerged site in poor visibility conditions. A performance analysis has been performed by comparing different image enhancement algorithms, and the results have been adopted to reconstruct an area of 40 m2 at a depth of about 5 m at the underwater archaeological site of Baiae (Italy).
Keywords: 3D reconstruction , Image enhancement , Underwater Cultural Heritage , Underwater imaging
-
A compared approach on how deep learning may support reverse engineering for tolerance inspection
ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 14
3D scanningGeometric dimensioning, tolerancing and inspection
Abstract
Reverse Engineering (RE) may help tolerance inspection during production by digitalization of analyzed components and their comparison with design requirements. RE techniques are already applied for geometrical and tolerance shape control. Plastic injection molding is one of the fields where it may be applied, in particular for die set-up of multi-cavities, since no severe accuracy is required for the acquisition system. In this field, RE techniques integrated with Computer-Aided tools for tolerancing and inspection may contribute to the so-called “Smart Manufacturingâ€. Their integration with PLM and suppliers' incoming components may set the information necessary to evaluate each component and die. Intensive application of shape digitalization has to front several issues: accuracy of data acquisition hardware and software; automation of experimental and post-processing steps; update of industrial protocol and workers knowledge among others. Concerning post-processing automation, many advantages arise from computer vision, considering that it is based on the same concepts developed in a RE post-processing (detection, segmentation and classification). Recently, deep learning has been applied to classify point clouds, considering object and/or feature recognition. This can be made in two ways: with a 3D voxel grid, increasing regularity, before feeding data to a deep net architecture; or acting directly on point cloud. Literature data demonstrate high accuracy according to net training quality. In this paper, a preliminary study about CNN for 3D points segmentation is provided. Their characteristics have been compared to an automatic approach that has been already implemented by the authors in the past. VoxNet and PointNet architectures have been compared according to the specific task of feature recognition for tolerance inspection and some investigations on test cases are discussed to understand their performance.
Keywords: Deep learning , Injection molding , PointNet , Reverse engineering , Tolerance inspection
-
Robust die compensation in sheet metal design through the integration of dual response surface and shape function optimization
Mathematical Problems in Engineering, 2019
Geometric dimensioning, tolerancing and inspectionProduct design and engineering
Abstract
In sheet metal forming, springback represents a major drawback increasing die set-up problems, especially for ultra-high strength steels. Finite Element Analysis is a well-established method to simulate the process during design, and multicriteria optimizations, for example, via surrogate models, are investigated in order to develop integrated design. Since to take into account also springback compensation die design may involve a large number of geometric variables, this paper presents a robust design formulation, based on the adoption of the shape function optimization, to describe springback in terms of weights directly associated to global shape variations of the die shape. Doing so, multicriteria optimization, which involves also die compensation, can be set up in a more intuitive approach, as requested in the preliminary steps of die design. After the introduction of the industrial problem, the mathematical formulation of the shape function optimization is presented together with its novel extension to Robust Design, which is based on the Dual Response Surface. Through a test case derived from the head part of a B-pillar, stamped from a Dual Phase sheet 1.5 mm thick, this novel extension investigates the effect of 6% variation from nominal values of initial yield stress and thickness. Results demonstrate the feasibility of the procedure, underlying that an optimal compensation may not be optimal in terms of process robustness.
-
Posture evaluation for fragment re-alignment of ancient bronze statues: The case study of the principe ellenistico
Lecture Notes in Mechanical Engineering, pp. 323-335
3D technologies for cultural heritagesVirtual prototyping and digital twin
Abstract
Interventions of ancient bronze statues restoration may last long periods, involving several activities from material and structural analysis to set-up of museum exhibitions, passing through reconstruction of fragments. In this paper, we describe procedures and methods used for evaluation of the current posture of “Principe Ellenisticoâ€. In fact, the statue seems to present some inaccuracies, in the fragments assembly, made during the last restoration activity (one of this effect is clearly observed in the spear inclination). The final aims are: (1) evaluation of differences among the postures before and after the last restoration; (2) recognition of the original fragments embedded in a previous restoration; and (3) the study of a possible better positioning of them. Methods applied are related to feature recognition on acquired point clouds, image analysis through control points and algorithms to find centerline of the elements that could need to be repositioned. In the final part, a concept design for a new inner-support is presented, giving the possibility to avoid assembly inaccuracies. Future developments are presented as the prospect of additive manufacturing the support, firstly with a FDM prototype and then through SLM or similar technologies.
Keywords: 3D-reconstruction , Bronze statue restoration , Cultural heritage , Feature recognition , Virtual prototyping
-
Geometrical modelling effects on FEA of colorectal surgery
Computer-Aided Design and Applications, 16(4), pp. 778-788
Methods for medical applicationsProduct design and engineering
Abstract
The research reported in this paper applies an explicit non-linear FEA solver to simulate the interaction between a clamp and a hyper-elastic material that aims to mimic the biological tissue of the colon. More in detail, the paper provides new results as a continuation of a previous works aimed at the evaluation of this solver to manage contact and dynamic loading on complex, multiple shapes. Results concern with the evaluation of the contact force during clamping, thus to the assessment of the force-feedback. The analysis is carried out on two geometries, using the hyper-elastic Mooney-Rivlin model for the mechanical behavior of the soft tissues. A pressure is applied on the colon to simulate the surgical clamp, which goes progressively in contact with tissue surface. To assess FEA criticality, and, then, its feasibility, the stress-strain and the contact force are analysed according to geometrical model and thickness variation, leaving the pressure constant. Doing so, their effect on the force-feedback can be foreseen, understanding their role on the accuracy of the final result.
Keywords: Computer assisted surgical planning , Finite element analysis , Segmentation , Soft tissues simulations
-
An integrated approach for shape optimization with mesh-morphing
Lecture Notes in Mechanical Engineering, pp. 311-322
Product design and engineering
Abstract
Although the CAD parameters allow to update easily the geometrical model, the numerical models updating into Finite Elements (FE) software with different mesh result to be often heavy, due to the necessity both to create new mesh and to make usually time consuming and complex CAE calculations for updating the loading conditions. The aim of the present research is to devise a reliable methodology and at the same time to reduce computational burden in the shape optimization studies of mechanical components. In particular, an integrated Multibody (MB) and Mesh-Morphing (MM) approach was developed to perform shape optimization, in order to reduce maximum tensions. Using the RBF Morph ACT Extension plugin implemented in the commercial solver FEM ANSYS® Mechanical vers. 18.2 along with the commercial MB software MSC ADAMS® vers. 2017, shape optimizations can be obtained in a very short time, by acting directly at the mesh so updating node positions and mesh elements geometry without bringing different geometrical models of the component into the FE environment. To validate the methodology, a crankshaft for a high performance Internal Combustion Engine (I.C.E.) was chosen, as case study, to optimize the fillet zones between web and pin.
Keywords: Crankshaft , FEA , Fillet zones , Multibody , Stress analysis
-
Pectus Carinatum: a non-invasive and objective measurement of severity
Medical and Biological Engineering and Computing, 57(8), pp. 1727-1735
3D scanningMethods for medical applications
Abstract
To date, standard methods for assessing the severity of chest wall deformities are mostly linked to X-ray and CT scans. However, the use of radiations limits their use when there is a need to monitor the development of the pathology over time. This is particularly important when dealing with patients suffering from Pectus Carinatum, whose treatment mainly requires the use of corrective braces and a systematic supervision. In recent years, the assessment of severity of chest deformities by means of radiation-free devices became increasingly popular but not yet adopted as standard clinical practice. The present study aims to define an objective measure by defining a severity index (named External Pectus Carinatum Index) used to monitor the course of the disease during treatment. Computed on the optical acquisition of the patients' chest by means of an appositely devised, fast and easy-to-use, body scanner, the proposed index has been validated on a sample composed of a control group and a group of Pectus Carinatum patients. The index proved to be reliable and accurate in the characterization of the pathology, enabling the definition of a threshold that allows to distinguish the cases of patients with PC from those of healthy subjects. [Figure not available: see fulltext.]
Keywords: Index , Optical imaging , Pectus Carinatum , Severity assessment , Three-dimensional
-
Design and production of innovative turbomachinery components via topology optimization and additive manufacturing
International Journal of Rotating Machinery, 2019
Product design and engineering
Abstract
The present paper proposes a methodology to design and manufacture optimized turbomachinery components by leveraging the potential of Topology Optimization (TO) and Additive Manufacturing (AM). The method envisages the use of TO to define the best configuration of the rotoric components in terms of both static and dynamic behavior with a resultant reduction of overall weight. Eventually, the topology-optimized component is manufactured by using appropriate materials that can guarantee valid mechanical performances. The proposed strategy has been applied to a 2D impeller used for centrifugal compressors to prove the effectiveness of a TO+AM-based approach. Although this approach has never been extensively used before to centrifugal compressors and expanders, its application on rotor and stator components might unlock several benefits: Tuning the natural frequencies, a reduction in the stress level, and a lighter weight of the rotating part. These objectives can be reached alone or in combination, performing a single analysis or a multiple analyses optimization. Finally, the introduction of AM technologies as standard manufacturing resources could bring sensible benefits with respect to the time to production and availability of components. Such aspects are essential in the Oil and Gas context, when dealing with new projects but also for service operations.
-
Novel design for a customized, 3D-printed surgical template for thoracic spinal arthrodesis
International Journal of Medical Robotics and Computer Assisted Surgery, 15(4)
Additive manufacturingMethods for medical applications
Abstract
Background: The integration of computer-aided design/computer-aided manufacturing (CAD/CAM) tools and medicine is rapidly developing for designing medical devices. A novel design for a 3D-printed patient-specific surgical template for thoracic pedicle screw insertion, using a procedure based on reverse engineering, is presented. Methods: The surgeon chooses the entry point on the vertebra. The optimal insertion direction and the size of the screws are defined via an algorithm on the basis of a patient-specific vertebra CAD model. The template features an innovative shape for a comfortable and univocal placement and a novel disengaging device. Results: Three spinal fusions were performed to test the template. Excellent results were achieved in terms of the accuracy of the screw positioning, reduction in surgery duration, and number of X-rays. Conclusions: A novel design for a customized, 3D-printed surgical template for thoracic spinal arthrodesis was presented, and improvements in terms of precision, duration, and safety were achieved without changing the standard procedure.
Keywords: 3D imaging , additive manufacturing , bone , computer-assisted surgery , imaged guided surgery , in vivo , modelling , pedicle screw fixation , screw direction optimization , spine , surgical template , thoracic , thoracic spinal arthrodesis , X-ray minimization
-
Meta-heuristic multidisciplinary design optimization of wind turbine blades obtained from circular pipes
Engineering with Computers, 35(2), pp. 363-379
Product design and engineering
Abstract
Aim of this paper is to present a methodology useful to optimize the geometry of the blades of a small-size wind turbine which are obtained from a circular pipe: an optimal chord distribution and airfoil sweep can be obtained with a proper cutting path. A strong reduction in manufacturing costs and time can be achieved for blades which are a critical element in wind turbine systems, especially in case of renewable plants in developing countries. An algorithm has been developed to obtain the shape of the blades and wind turbine performances are computed by the Blade-Element Method, due to its low computational simplicity; the XFoil tool has been used to compute the aerodynamic of the blades. Heuristic algorithms have been applied to obtain a feasible design solution assuring the best efficiency of the wind turbine. Also structural considerations are kept into account to provide a feasible configuration able to withstand the forces acting on the rotating blades. Results obtained suggest that an optimal design of such a kind of blades can be obtained thanks to this methodology. The mathematical framework developed for the optimization is efficient and the heuristics algorithms allow the convergence to feasible configurations. The computing time is compatible with a practical application of the method also in industries.
Keywords: CAD , Design , Multidisciplinary optimization , Particle swarm algorithm , Wind turbine
-
A design approach to support BIM for existing structures
Lecture Notes in Mechanical Engineering, pp. 292-300
Product design and engineering
Abstract
Nowadays, Building Information Modeling (BIM) is a common design approach to support the life cycle of projects in the field of Architecture Engineering Construction (AEC). New constructions' projects require a BIM modeling to provide digital information within a 3D digital mockup. The main target is to reduce time and cost related to the elaboration of additional and not integrated documentation. This issue is also common in projects focused on the renovations of existing buildings. In fact, the BIM approach provides tools to improve interoperability between different software to integrate analysis and simulations within the architectural representation. The renovation projects require reverse engineering tools and methods for the 3D modeling of existing structures. One of the issues concerns the digital photogrammetric survey of glass surfaces. This paper proposes a design approach to support BIM phases for already existing structures with a test case focused on a hallway with a continuous glass wall.
Keywords: BIM , Photo matching , Photo survey , Steel-glass buildings
-
Replicas fabrication by laser scanner and additive manufacturing: A preliminary investigation
ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 14
3D scanningAdditive manufacturingDesign methods for mobilityVirtual prototyping and digital twin
Abstract
This paper presents a preliminary investigation on the workflow that allows to replicate object by using 3D laser scanner and a desktop fused filament fabrication 3D printer. Pitfalls and limitations of those technologies will be pointed out in order to find the bottleneck of the workflow, paying specific attention to what concerns the digital workflow from the acquisition to the generation of the g-code. The findings and conclusions are drawn from a case study that has been carried out using the minimum amount of human intervention, especially during the digital post-processing of the data. The objects under investigation is a broken car door handle. Firstly, it has been digitalized using a 3D laser scanner properly calibrated and set. The accuracy, precision and resolution of the measurement tool have been recorded and the as-is acquired data has been checked against topological errors. The as-is acquired model has been compared with the original geometry. The 3d polygonal mesh has been prepared for being printed: the material, machine and process parameters have been chosen. A simulation of the deposition process to estimate warps and deviation from the nominal geometry was carried out. Finally, the object has been additively manufactured using a desktop Fused Filament Fabrication machine: the printed object has been again compared with the original geometry.
-
Design for Powder Metallurgy: Predicting Anisotropic Dimensional Change on Sintering of Real Parts
International Journal of Precision Engineering and Manufacturing, 20(4), pp. 619-630
Product design and engineering
Abstract
Anisotropic dimensional change on sintering may strongly affect the precision of parts produced by press and sinter. In previous work a design procedure accounting for anisotropic dimensional change of axi-symmetric parts (disks and rings) has been developed on the basis of experimental data. In this work the procedure has been applied to predict the anisotropic dimensional change of real parts produced in industrial conditions, providing that coaxial rings were identified in the geometry of the actual parts. Parts were highly different for material, complexity of geometry, green density and process conditions. Parts were measured in the green and sintered state and the measured dimensional changes were compared to the predicted ones, finding a good agreement. The procedure was also adapted to predict dimensional change of an oval feature, and highly satisfactory results were obtained.
Keywords: Anisotropy , Dimensional change , Powder metallurgy , Precision of PM parts
-
Do bioreactor designs with more efficient oxygen supply to ovarian cortical tissue fragments enhance follicle viability and growth in vitro?
Processes, 7(7)
Methods for medical applicationsProduct design and engineering
Abstract
Background: Autotransplantation of cryopreserved ovarian tissue is currently the main option to preserve fertility for cancer patients. To avoid cancer cell reintroduction at transplantation, amulti-step culture systemhas been proposed to obtain fully competent oocytes for in vitro fertilization. Current in vitro systems are limited by the low number and health of secondary follicles produced during the first step culture of ovarian tissue fragments. To overcome such limitations, bioreactor designs have been proposed to enhance oxygen supply to the tissue, with inconsistent results. This retrospective study investigates, on theoretical grounds, whether the lack of a rational design of the proposed bioreactors prevented the full exploitation of follicle growth potential. Methods: Models describing oxygen transport in bioreactors and tissue were developed and used to predict oxygen availability inside ovarian tissue in the pertinent literature. Results: The proposed theoretical analysis suggests that a successful outcome is associated with enhanced oxygen availability in the cultured tissue in the considered bioreactor designs. This suggests that a rational approach to bioreactor design for ovarian tissue culture in vitro may help exploit tissue potential to support follicle growth.
Keywords: Design , In vitro culture , Ioreactor , Ovarian tissue , Oxygen , Transport